Source : |
HEK293 |
Species : |
Cynomolgus/Rhesus macaque |
Tag : |
lFc |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : |
Immobilized Cynomolgus / Rhesus macaque BCMA, Llama IgG2b Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) with a linear range of 0.1-8 ng/mL.Immobilized Human APRIL, His,Flag Tag, active trimer at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque BCMA, Llama IgG2b Fc Tag with a linear range of 0.1-8 ng/mL. |
Molecular Mass : |
This protein carries a llama IgG2b Fc tag at the C-terminusThe protein has a calculated MW of 33.8 kDa. The protein migrates as 40-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Met 1-Ala 53 |
Endotoxin : |
Less than 0.01 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >95% as determined by SEC-MALS. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |