| Source : |
HEK293 |
| Species : |
Cynomolgus |
| Tag : |
His-Flag |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Cynomolgus APRIL, His,Flag Tag at 1 μg/mL (100 μL/well) can bind Human TACI (R119A, R122A), Fc Tag with a linear range of 2-13 ng/mL.Immobilized Cynomolgus APRIL, His,Flag Tag at 1 μg/mL (100 μL/well) can bind Human BCMA, Fc Tag (HPLC verified) with a linear range of 0.1-1 ng/mL. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.The protein has a calculated MW of 50.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Gln 111-Leu 250 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
