| Species : |
Dog |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
24-132 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
The interleukin 4 (IL4,) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. The activity of IL4 is usually measured by a cell proliferation assay using TF-1 cells. TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20000 cells/well with 10% serum standard 1640 which contains various concentrations of recombinant human IL4. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37°C. Proliferation of TF-1 cells after incubation with IL4 for 3 days observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with rhIL4 for 3 days. It was obvious that rhIL4 significantly increased cell viability of TF-1 cells. The ED50 is 4.2 ng/ml. |
| Molecular Mass : |
14.4kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
