Active Recombinant Dog PDE4D Protein (2-679), N-GST tagged

• Cat.No.: PDE4D-11D
• Product Overview: Dog phosphodiesterase 4D (PDE4D) GenBank Accession No. XP_003433853, a.a. 2-679 (end), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Specification

• Description: Enables beta-2 adrenergic receptor binding activity; cyclic-nucleotide phosphodiesterase activity; and enzyme binding activity. Involved in several processes, including positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; and regulation of signal transduction. Located in several cellular components, including Golgi apparatus; centrosome; and myofibril. Part of protein-containing complex. Used to study mental depression. Biomarker of transient cerebral ischemia. Human ortholog(s) of this gene implicated in acrodysostosis. Orthologous to human PDE4D (phosphodiesterase 4D).
• Source: Sf9 insect cells
• Species: Dog
• Tag: N-GST
• Form: Aqueous buffer solution
• Bio-activity: ≥ 5665 pmol/min/μg
• Molecular Mass: 104 kDa
• Protein Length: 2-679
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37 centigrade.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage Buffer: 40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20 % glycerol, 3 mM DTT
• Shipping: At -80 centigrade
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: PDE4D phosphodiesterase 4D [ Canis lupus familiaris (dog) ]
• Official Symbol: PDE4D
• Synonyms: Pde4d; phosphodiesterase 4D; DPDE3; PDE3/IVd; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; 3',5'-cyclic AMP phosphodiesterase; cAMP-specific phosphodiesterase PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); EC 3.1.4.53
• Gene ID: 487221
• mRNA Refseq: XM_003433805
• Protein Refseq: XP_003433853
• UniProt ID: F1PKQ9