Active Recombinant Full Length Cynomolgus caspase 3 Protein, His-tagged

• Cat.No.: CASP3-363CFL
• Product Overview: Active Recombinant Full Length Cynomolgus CASP3 Protein with C His-tag was expressed in E. coli.

Specification

• Description: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.
• Source: E. coli
• Species: Cynomolgus
• Tag: C-His
• Protein length: Full Length
• Bio-activity: 6.52×10^8 U/mg protein. One unit is the amount of enzyme that will cleave 1.0 nmol of the colorimetric substrate Ac-DEVD-pNA per hour at 37 centigrade under saturated substrate concentrations.
• Molecular Mass: 32 kDa
• AA Sequence: MENTENSVDSKSIKSLEPKIIHGSK SVDSGISLDNSYKMDYPEMGLCIII NNKNFHKSTGMASRSGTDVDAANLR ETFINLKYGVRNKNDLTREEIVELM RNVSKEDHSKRSSFVCVLLSHGEEG IIFGTNGPLDLKKITSFFRGDCCRS LTGKPKLFIIQACRGTELDCGIEAD SGVEDDMACHKIPVEADFLYAYSTA PGYYSWRNSKDGSWFIQSLCAMLKQ YADKLEFMHILTRVNRKVATEFESF SLDATFHAKKQIPCIVSMLTKELYF YHHHHHHHH
• Purity: > 90 % by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 2 mg/mL by BCA
• Storage Buffer: Sterile 50mM Tris, 300mM NaCl, pH8.0, 200mM Arginine

Gene Information

• Gene Name: CASP3 caspase 3 [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: CASP3
• Synonyms: CASP3; caspase 3; caspase-3; caspase 3, apoptosis-related cysteine peptidase; caspase-3 preproprotein; EC 3.4.22.56
• Gene ID: 694473
• mRNA Refseq: XM_015139582
• Protein Refseq: XP_014995068
• UniProt ID: F7HFY2

Reviews

03/29/2024

Casp3-748R, the rat caspase3 worked/is working very well for us.