Active Recombinant Full Length Human ACTN4 Protein, C-Flag-tagged

• Cat.No.: ACTN4-390HFL
• Product Overview: Recombinant Full Length Human ACTN4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: WB positive control
• Molecular Mass: 104.7 kDa
• AA Sequence: MVDYHAANQSYQYGPSSAGNGAGGG GSMGDYMAQEDDWDRDLLLDPAWEK QQRKTFTAWCNSHLRKAGTQ IENIDEDFRDGLKLMLLLEVISGER LPKPERGKMRVHKINNVNKALDFIA SKGVKLVSIGAEEIVDGNAK MTLGMIWTIILRFAIQDISVEETSA KEGLLLWCQRKTAPYKNVNVQNFHI SWKDGLAFNALIHRHRPELI EYDKLRKDDPVTNLNNAFEVAEKYL DIPKMLDAEDIVNTARPDEKAIMTY VSSFYHAFSGAQKAETAANR ICKVLAVNQENEHLMEDYEKLASDL LEWIRRTIPWLEDRVPQKTIQEMQQ KLEDFRDYRRVHKPPKVQEK CQLEINFNTLQTKLRLSNRPAFMPS EGKMVSDINNGWQHLEQAEKGYEEW LLNEIRRLERLDHLAEKFRQ KASIHEAWTDGKEAMLKHRDYETAT LSDIKALIRKHEAFESDLAAHQDRV EQIAAIAQELNELDYYDSHN VNTRCQKICDQWDALGSLTHSRREA LEKTEKQLEAIDQLHLEYAKRAAPF NNWMESAMEDLQDMFIVHTI EEIEGLISAHDQFKSTLPDADRERE AILAIHKEAQRIAESNHIKLSGSNP YTTVTPQIINSKWEKVQQLV PKRDHALLEEQSKQQSNEHLRRQFA SQANVVGPWIQTKMEEIGRISIEMN GTLEDQLSHLKQYERSIVDY KPNLDLLEQQHQLIQEALIFDNKHT NYTMEHIRVGWEQLLTTIARTINEV ENQILTRDAKGISQEQMQEF RASFNHFDKDHGGALGPEEFKACLI SLGYDVENDRQGEAEFNRIMSLVDP NHSGLVTFQAFIDFMSRETT DTDTADQVIASFKVLAGDKNFITAE ELRRELPPDQAEYCIARMAPYQGPD AVPGALDYKSFSTALYGESD
  LTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction

Gene Information

• Gene Name: ACTN4 actinin alpha 4 [ Homo sapiens (human) ]
• Official Symbol: ACTN4
• Synonyms: FSGS; FSGS1; ACTININ-4
• Gene ID: 81
• mRNA Refseq: NM_004924.6
• Protein Refseq: NP_004915.2
• MIM: 604638
• UniProt ID: O43707