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Active Recombinant Full Length Human ARG2 Protein, C-Flag-tagged

Cat.No. : ARG2-148HFL
Product Overview : Recombinant Full Length Human ARG2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.
  • Specification
  • Gene Information
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Description : Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described.
Source : Mammalian cells
Species : Human
Tag : Flag
Form : 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bio-activity : L-Arginase activity verified in a biochemical assay: Arginase 2 activity was measured in a colorimetric biochemical assay. Arginase 1 catalyzes the conversion of arginine to ornithine and urea. After incubation of the protein in a solution containing arginine, the reaction is stopped, and the urea concentration is measured by a chemical reaction that produces a colored product that absorbs at 430 nm. By measuring the absorbance at 430 nm and comparing to a standard, the specific activity of this preparation of ARG2 was calculated to be approximately 10U/mg.
Unit definition: 1 unit of ARG2 converts 1 µmole of L-arginine to ornithine and urea per minute at pH 9.5 and 37 centigrade.
Molecular Mass : 36 kDa
AA Sequence : MSLRGSLSRLLQTRVHSILKKSVHS VAVIGAPFSQGQKRKGVEHGPAAIR EAGLMKRLSSLGCHLKDFGD LSFTPVPKDDLYNNLIVNPRSVGLA NQELAEVVSRAVSDGYSCVTLGGDH SLAIGTISGHARHCPDLCVV WVDAHADINTPLTTSSGNLHGQPVS FLLRELQDKVPQLPGFSWIKPCISS ASIVYIGLRDVDPPEHFILK NYDIQYFSMRDIDRLGIQKVMERTF DLLIGKRQRPIHLSFDIDAFDPTLA PATGTPVVGGLTYREGMYIA EEIHNTGLLSALDLVEVNPQLATSE EEAKTTANLAVDVIASSFGQTREGG HIVYDQLPTPSSPDESENQA
RVRITRTRPLEQKLISEEDLAANDI LDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining.
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade.
Concentration : >50 ug/mL as determined by microplate BCA method.
Preparation : Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Pathways : Arginine and proline metabolism, Metabolic pathways
Gene Name : ARG2 arginase 2 [ Homo sapiens (human) ]
Official Symbol : ARG2
Synonyms : arginase 2; kidney arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase
Gene ID : 384
mRNA Refseq : NM_001172.4
Protein Refseq : NP_001163.1
MIM : 107830
UniProt ID : P78540

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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