Active Recombinant Full Length Human BMX Protein, C-Flag-tagged

• Cat.No.: BMX-462HFL
• Product Overview: Recombinant Full Length Human BMX Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Alternatively spliced transcript variants have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: BMX activity verified in a biochemical assay: BMX (BMX non-receptor tyrosine kinase) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. BMX is a non-receptor tyrosine kinase belonging to the Tec kinase family. BMX has been implicated in the Stat pathway and reportedly regulates differentiation and tumorigenicity of several types of cancer cells. Varying concentrations of BMX were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
• Molecular Mass: 77.8 kDa
• AA Sequence: MDTKSILEELLLKRSQQKKKMSPNN YKERLFVLTKTNLSYYEYDKMKRGS RKGSIEIKKIRCVEKVNLEE QTPVERQYPFQIVYKDGLLYVYASN EESRSQWLKALQKEIRGNPHLLVKY HSGFFVDGKFLCCQQSCKAA PGCTLWEAYANLHTAVNEEKHRVPT FPDRVLKIPRAVPVLKMDAPSSSTT LAQYDNESKKNYGSQPPSSS TSLAQYDSNSKKIYGSQPNFNMQYI PREDFPDWWQVRKLKSSSSSEDVAS SNQKERNVNHTTSKISWEFP ESSSSEEEENLDDYDWFAGNISRSQ SEQLLRQKGKEGAFMVRNSSQVGMY TVSLFSKAVNDKKGTVKHYH VHTNAENKLYLAENYCFDSIPKLIH YHQHNSAGMITRLRHPVSTKANKVP DSVSLGNGIWELKREEITLL KELGSGQFGVVQLGKWKGQYDVAVK MIKEGSMSEDEFFQEAQTMMKLSHP KLVKFYGVCSKEYPIYIVTE YISNGCLLNYLRSHGKGLEPSQLLE MCYDVCEGMAFLESHQFIHRDLAAR NCLVDRDLCVKVSDFGMTRY VLDDQYVSSVGTKFPVKWSAPEVFH YFKYSSKSDVWAFGILMWEVFSLGK QPYDLYDNSQVVLKVSQGHR
  LYRPHLASDTIYQIMYSCWHELPEK RPTFQQLLSSIEPLREKDKHTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: BMX BMX non-receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: BMX
• Synonyms: ETK; PSCTK2; PSCTK3
• Gene ID: 660
• mRNA Refseq: NM_001721.7
• Protein Refseq: NP_001712.1
• MIM: 300101
• UniProt ID: P51813