Active Recombinant Full Length Human DNMT3A Protein, Flag-tagged

• Cat.No.: DNMT3A-01HFL
• Product Overview: Recombinant Full Length human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 3, fused with C-terminal Flag tag, was expressed in mamanlian cells.

Specification

• Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
• Source: Mamanlian cells
• Species: Human
• Tag: Flag
• Bio-activity: DNMT3A activity verified in a biochemical assay: DNMT3A (DNA (cytosine-5-)-methyltransferase 3 alpha) is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Varying concentrations of DNMT3A were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured.
  Surface Plasmon Ressonance (SPR)
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method.
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: DNMT3A DNA methyltransferase 3 alpha [ Homo sapiens (human) ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA methyltransferase 3 alpha; TBRS; HESJAS; DNMT3A2; M.HsaIIIA; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; cysteine methyltransferase DNMT3A; EC 2.1.1.37
• Gene ID: 1788
• mRNA Refseq: NM_022552
• Protein Refseq: NP_072046
• MIM: 602769
• UniProt ID: Q9Y6K1