Active Recombinant Full Length Human ERN1 Protein, C-Flag-tagged

• Cat.No.: ERN1-80HFL
• Product Overview: Recombinant Full Length Human ERN1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: WB positive control
  Binding assay (ITC)
• Molecular Mass: 109.6 kDa
• AA Sequence: MPARRLLLLLTLLLPGLGIFGSTST VTLPETLLFVSTLDGSLHAVSKRTG SIKWTLKEDPVLQVPTHVEE PAFLPDPNDGSLYTLGSKNNEGLTK LPFTIPELVQASPCRSSDGILYMGK KQDIWYVIDLLTGEKQQTLS SAFADSLCPSTSLLYLGRTEYTITM YDTKTRELRWNATYFDYAASLPEDD VDYKMSHFVSNGDGLVVTVD SESGDVLWIQNYASPVVAFYVWQRE GLRKVMHINVAVETLRYLTFMSGEV GRITKWKYPFPKETEAKSKL TPTLYVGKYSTSLYASPSMVHEGVA VVPRGSTLPLLEGPQTDGVTIGDKG ECVITPSTDVKFDPGLKSKN KLNYLRNYWLLIGHHETPLSASTKM LERFPNNLPKHRENVIPADSEKKSF EEVINLVDQTSENAPTTVSR DVEEKPAHAPARPEAPVDSMLKDMA TIILSTFLLIGWVAFIITYPLSMHQ QQQLQHQQFQKELEKIQLLQ QQQQQLPFHPPGDTAQDGELLDTSG PYSESSGTSSPSTSPRASNHSLCSG SSASKAGSSPSLEQDDGDEE TSVVIVGKISFCPKDVLGHGAEGTI VYRGMFDNRDVAVKRILPECFSFAD REVQLLRESDEHPNVIRYFC TEKDRQFQYIAIELCAATLQEYVEQ KDFAHLGLEPITLLQQTTSGLAHLH SLNIVHRDLKPHNILISMPN AHGKIKAMISDFGLCKKLAVGRHSF SRRSGVPGTEGWIAPEMLSEDCKEN PTYTVDIFSAGCVFYYVISE GSHPFGKSLQRQANILLGACSLDCL HPEKHEDVIARELIEKMIAMDPQKR PSAKHVLKHPFFWSLEKQLQ FFQDVSDRIEKESLDGPIVKQLERG GRAVVKMDWRENITVPLQTDLRKFR TYKGGSVRDLLRAMRNKKHH YRELPAEVRETLGSLPDDFVCYFTS RFPHLLAHTYRAMELCSHERLFQPY YFHEPPEPQPPVTPDALTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Protein Kinase, Transmembrane
• Protein Pathways: Alzheimer's disease

Gene Information

• Gene Name: ERN1 endoplasmic reticulum to nucleus signaling 1 [ Homo sapiens (human) ]
• Official Symbol: ERN1
• Synonyms: IRE1; IRE1P; IRE1a; hIRE1p
• Gene ID: 2081
• mRNA Refseq: NM_001433.5
• Protein Refseq: NP_001424.3
• MIM: 604033
• UniProt ID: O75460