| Species : |
Human |
| Source : |
293T |
| Tag : |
Non |
| Description : |
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. |
| Form : |
Cell-Tissue Lysis buffer |
| Molecular Mass : |
72 kDa |
| Notes : |
Instruction of use: This knockdown cell lysate should be paired with wild-type 293T cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins. |
| Storage : |
Store at -20 centigrade for two years. |
| Concentration : |
Lot-specific |
| Shipping : |
Blue Ice |
| Components : |
1 vial of 100 μg WT 293T cell lysate
1 vial of 100 μg HSPA5 KD 293T cell lysate |
| Protein Families : |
Druggable Genome |
| Protein Pathways : |
Antigen processing and presentation, Prion diseases |
| Lysate QC : |
RT-qPCR; Western Blotting (WB) |
