Active Recombinant Full Length Human MAP2K5 Protein, GST-tagged

• Cat.No.: MAP2K5-2401HF
• Product Overview: Full-length human MAP2K5 [1-448(end) amino acids of accession number NP_660143.1] was co- expressed as N-terminal GST-fusion protein (77 kDa) with human His-tagged MAP3K3[1-626(end) amino acids of accession number NP_002392.2], CDC37 and HSP90 using baculovirus expression system. GST-MAP2K5 was purified by using glutathione sepharose chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-448
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .
• Purity: 51%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase kinase 5
• Official Symbol: MAP2K5
• Synonyms: MAP2K5;HsT17454;MAPKK5;MEK5;PRKMK5
• Protein Refseq: NP_660143.1
• UniProt ID: Q13163