Active Recombinant Full Length Human MAPK10 Protein, GST-tagged

Cat.No. : MAPK10-8439HF
Product Overview : Full-length human JNK3 [1-426(end) amino acids of accession number NP_620446.1] was expressed as N-terminal GST-fusion protein (75 kDa) using E.coli expression system. GST-JNK3 was purified by using glutathione sepharose chromatography and activated with His-tagged MAP2K4 and MAP2K7. Activated GST-JNK3 was purified using glutathione sepharose chromatography.
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Source : E. coli
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Protein length : 1-426
Purity : 86%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
SDS-PAGE: The purity was assessed by SDS-PAGE/CBB staining.
Gene Name : mitogen-activated protein kinase 10
Official Symbol : MAPK10
Synonyms : MAPK10;JNK3;JNK3A;PRKM10;SAPK1b;p493F12;p54bSAPK
Protein Refseq : NP_620446.1
UniProt ID : P53779

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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