Active Recombinant Full Length Human NASP Protein, C-Flag-tagged

• Cat.No.: NASP-585HFL
• Product Overview: Recombinant Full Length Human NASP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme substrate
• Molecular Mass: 85.1 kDa
• AA Sequence: MAMESTATAAVAAELVSADKIEDVP APSTSADKVESLDVDSEAKKLLGLG QKHLVMGDIPAAVNAFQEAA SLLGKKYGETANECGEAFFFYGKSL LELARMENGVLGNALEGVHVEEEEG EKTEDESLVENNDNIDEEAR EELREQVYDAMGEKEEAKKTEDKSL AKPETDKEQDSEMEKGGREDMDISK SAEEPQEKVDLTLDWLTETS EEAKGGAAPEGPNEAEVTSGKPEQE VPDAEEEKSVSGTDVQEECREKGGQ EKQGEVIVSIEEKPKEVSEE QPVVTLEKQGTAVEVEAESLDPTVK PVDVGGDEPEEKVVTSENEAGKAVL EQLVGQEVPPAEESPEVTTE AAEASAVEAGSEVSEKPGQEAPVLP KDGAVNGPSVVGDQTPIEPQTSIER LTETKDGSGLEEKVRAKLVP SQEETKLSVEESEAAGDGVDTKVAQ GATEKSPEDKVQIAANEETQEREEQ MKEGEETEGSEEDDKENDKT EEMPNDSVLENKSLQENEEEEIGNL ELAWDMLDLAKIIFKRQETKEAQLY AAQAHLKLGEVSVESENYVQ AVEEFQSCLNLQEQYLEAHDRLLAE THYQLGLAYGYNSQYDEAVAQFSKS IEVIENRMAVLNEQVKEAEG SSAEYKKEIEELKELLPEIREKIED AKESQRSGNVAELALKATLVESSTS GFTPGGGGSSVSMIASRKPT DGASSSNCVTDISHLVRKKRKPEEE SPRKDDAKKAKQEPEVNGGSGDAVP SGNEVSENMEEEAENQAESR
  AAVEGTVEAGATVESTACTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: NASP nuclear autoantigenic sperm protein [ Homo sapiens (human) ]
• Official Symbol: NASP
• Synonyms: HMDRA1; FLB7527; PRO1999
• Gene ID: 4678
• mRNA Refseq: NM_002482.4
• Protein Refseq: NP_002473.2
• MIM: 603185
• UniProt ID: P49321