Active Recombinant Full Length Human NIT2 Protein, C-Flag-tagged

• Cat.No.: NIT2-243HFL
• Product Overview: Recombinant Full Length Human NIT2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme activity
• Molecular Mass: 30.4 kDa
• AA Sequence: MTSFRLALIQLQISSIKSDNVTRAC SFIREAATQGAKIVSLPECFNSPYG AKYFPEYAEKIPGESTQKLS EVAKECSIYLIGGSIPEEDAGKLYN TCAVFGPDGTLLAKYRKIHLFDIDV PGKITFQESKTLSPGDSFST FDTPYCRVGLGICYDMRFAELAQIY AQRGCQLLVYPGAFNLTTGPAHWEL LQRSRAVDNQVYVATASPAR DDKASYVAWGHSTVVNPWGEVLAKA GTEEAIVYSDIDLKKLAEIRQQIPV FRQKRSDLYAVEMKKPTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: NIT2 nitrilase family member 2 [ Homo sapiens (human) ]
• Official Symbol: NIT2
• Synonyms: HEL-S-8a
• Gene ID: 56954
• mRNA Refseq: NM_020202.5
• Protein Refseq: NP_064587.1
• MIM: 616769
• UniProt ID: Q9NQR4