Active Recombinant Full Length Human NR1H3 Protein, C-Flag-tagged

• Cat.No.: NR1H3-464HFL
• Product Overview: Recombinant Full Length Human NR1H3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Binding assay (Blue Native PAGE)
• Molecular Mass: 50.2 kDa
• AA Sequence: MSLWLGAPVPDIPPDSAVELWKPGA QDASSQAQGGSSCILREEARMPHSA GGTAGVGLEAAEPTALLTRA EPPSEPTEIRPQKRKKGPAPKMLGN ELCSVCGDKASGFHYNVLSCEGCKG FFRRSVIKGAHYICHSGGHC PMDTYMRRKCQECRLRKCRQAGMRE ECVLSEEQIRLKKLKRQEEEQAHAT SLPPRASSPPQILPQLSPEQ LGMIEKLVAAQQQCNRRSFSDRLRV TPWPMAPDPHSREARQQRFAHFTEL AIVSVQEIVDFAKQLPGFLQ LSREDQIALLKTSAIEVMLLETSRR YNPGSESITFLKDFSYNREDFAKAG LQVEFINPIFEFSRAMNELQ LNDAEFALLIAISIFSADRPNVQDQ LQVERLQHTYVEALHAYVSIHHPHD RLMFPRMLMKLVSLRTLSSV
  HSEQVFALRLQDKKLPPLLSEIWDV HETRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: PPAR signaling pathway

Gene Information

• Gene Name: NR1H3 nuclear receptor subfamily 1 group H member 3 [ Homo sapiens (human) ]
• Official Symbol: NR1H3
• Synonyms: LXRA; LXR-a; RLD-1
• Gene ID: 10062
• mRNA Refseq: NM_005693.4
• Protein Refseq: NP_005684.2
• MIM: 602423
• UniProt ID: Q13133