Active Recombinant Full Length Human PRKCE Protein, GST-tagged

• Cat.No.: PRKCE-3146HF
• Product Overview: Full-length human PKCε [1-737(end) amino acids of accession number NP_005391.1] was expressed as N-terminal GST-fusion protein (111 kDa) using baculovirus expression system. GST-PKCε was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Lipid Activator. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-737
• Purity: 85%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase C, epsilon
• Official Symbol: PRKCE
• Synonyms: PRKCE;PKCE;nPKC-epsilon
• Protein Refseq: NP_005391.1
• UniProt ID: Q02156