Active Recombinant Full Length Human PRKCQ Protein, GST-tagged

• Cat.No.: PRKCQ-5515HF
• Product Overview: Full-length human PKCθ [1-706(end) amino acids of accession number NP_006248.1] was expressed as N-terminal GST-fusion protein (109 kDa) using baculovirus expression system. GST-PKCθ was purified by using glutathione sepharose chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-706
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Lipid Activator. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Purity: 73%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase C, theta
• Official Symbol: PRKCQ
• Synonyms: PRKCQ;PRKCT;nPKC-theta
• Protein Refseq: NP_006248.1
• UniProt ID: Q04759