| Species : |
Human |
| Source : |
Bacteria |
| Tag : |
His |
| Protein Length : |
1-192 aa |
| Description : |
This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. |
| Form : |
lyophilized powder |
| Molecular Weight : |
22 kDa |
| Bio-Activity : |
The biological activity is determined by its ability to exchange nucleotide. This is tested by a pulldown assay using GST tagged PAK-PBD beads and GTPγS or GDP loaded His-Rac2. The PAK protein is an effector of Rac2, and will specifically bind to the GTP bound form Rac2. Using this assay, the amount of biologically active GTP-bound Rac2 is determined. Stringent quality control ensures that >80% of the His-Rac2 protein produced is capable of binding GTP. |
| Purity : |
> 90% |
| Applications : |
Rac biochemistry
Rac2 GTPase assays
Rac2 nucleotide exchange assays
Rac2 binding studies |
| Storage Buffer : |
2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran |
| Reconstitution : |
In distilled water to 1 mg/mL. |
