Active Recombinant Full Length Human RAC2 Protein, His tagged

• Cat.No.: RAC2-100HFL
• Product Overview: The human Rac2 protein has been produced in a bacterial expression system.

Specification

• Species: Human
• Source: Bacteria
• Tag: His
• Protein Length: 1-192 aa
• Description: This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6.
• Form: lyophilized powder
• Molecular Weight: 22 kDa
• Bio-Activity: The biological activity is determined by its ability to exchange nucleotide. This is tested by a pulldown assay using GST tagged PAK-PBD beads and GTPγS or GDP loaded His-Rac2. The PAK protein is an effector of Rac2, and will specifically bind to the GTP bound form Rac2. Using this assay, the amount of biologically active GTP-bound Rac2 is determined. Stringent quality control ensures that >80% of the His-Rac2 protein produced is capable of binding GTP.
• Purity: > 90%
• Applications: Rac biochemistry Rac2 GTPase assays Rac2 nucleotide exchange assays Rac2 binding studies
• Storage Buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran
• Reconstitution: In distilled water to 1 mg/mL.

Gene Information

• Gene Name: RAC2 Rac family small GTPase 2 [ Homo sapiens (human) ]
• Official Symbol: RAC2
• Synonyms: RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); ras-related C3 botulinum toxin substrate 2; EN 7; p21-Rac2; small G protein; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); Gx; EN-7; HSPC022
• Gene ID: 5880
• mRNA Refseq: NM_002872
• Protein Refseq: NP_002863
• MIM: 602049
• UniProt ID: P15153