Active Recombinant Full Length Human RUNX1 Protein, C-Flag-tagged

• Cat.No.: RUNX1-188HFL
• Product Overview: Recombinant Full Length Human RUNX1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: RUNX1 Activity verified in a DNA-binding assay
• Molecular Mass: 48.6 kDa
• AA Sequence: MRIPVDASTSRRFTPPSTALSPGKM SEALPLGAPDAGAALAGKLRSGDRS MVEVLADHPGELVRTDSPNF LCSVLPTHWRCNKTLPIAFKVVALG DVPDGTLVTVMAGNDENYSAELRNA TAAMKNQVARFNDLRFVGRS GRGKSFTLTITVFTNPPQVATYHRA IKITVDGPREPRRHRQKLDDQTKPG SLSFSERLSELEQLRRTAMR VSPHHPAPTPNPRASLNHSTAFNPQ PQSQMQDTRQIQPSPPWSYDQSYQY LGSIASPSVHPATPISPGRA SGMTTLSAELSSRLSTAPDLTAFSD PRQFPALPSISDPRMHYPGAFTYSP TPVTSGIGIGMSAMGSATRY HTYLPPPYPGSSQAQGGPFQASSPS YHLYYGASAGSYQFSMVGGERSPPR ILPPCTNASTGSALLNPSLP
  NQSDVVEAEGSHSNSPTNMAPSARL EEAVWRPYTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Chronic myeloid leukemia, Pathways in cancer

Gene Information

• Gene Name: RUNX1 RUNX family transcription factor 1 [ Homo sapiens (human) ]
• Official Symbol: RUNX1
• Synonyms: AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
• Gene ID: 861
• mRNA Refseq: NM_001001890.3
• Protein Refseq: NP_001001890.1
• MIM: 151385
• UniProt ID: Q01196