Active Recombinant Full Length Human sialic acid acetylesterase protein, His tagged

• Cat.No.: SIAE-10HFL
• Product Overview: Recombinant Full Length Human sialic acid acetylesterase protein with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 1-523 aa
• Description: Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.
• Bio-activity: Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is > 25000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 57 kDa
• N-terminal Sequence Analysis: Ile24
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Shipping: The product is shipped with polar packs.
• Reference: 1. Higa, H.H. et. al. (1987) Biochem. Biophys. Res. Commun. 144:1099.
  2. Orizio, F. et. al. (2015) Glycobiology 9:992.
  3. Zhu, H. et. al. (2004) J. Biomed. Biotechnol. 3:130.

Gene Information

• Gene Name: SIAE sialic acid acetylesterase [ Homo sapiens (human) ]
• Official Symbol: SIAE
• Synonyms: SIAE; sialic acid acetylesterase; YSG2, Ysg2 homolog (mouse); sialate O-acetylesterase; CSE C; LSE; MGC87009; sialic acid specific acetylesterase II; H-Lse; sialic acid-specific acetylesterase II; cytosolic sialic acid 9-O-acetylesterase homolog; AIS6; CSEC; YSG2; CSE-C
• Gene ID: 54414
• mRNA Refseq: NM_001199922
• Protein Refseq: NP_001186851
• MIM: 610079
• UniProt ID: Q9HAT2