Active Recombinant Full Length Human SLC6A4 Protein, C-Flag-tagged

• Cat.No.: SLC6A4-601HFL
• Product Overview: Recombinant Full Length Human SLC6A4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake. There have been conflicting results in the literature about the possible effect, if any, that this polymorphism may play in behavior and depression.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: WB standard
• Molecular Mass: 70.1 kDa
• AA Sequence: METTPLNSQKQLSACEDGEDCQENG VLQKVVPTPGDKVESGQISNGYSAV PSPGAGDDTRHSIPATTTTL VAELHQGERETWGKKVDFLLSVIGY AVDLGNVWRFPYICYQNGGGAFLLP YTIMAIFGGIPLFYMELALG QYHRNGCISIWRKICPIFKGIGYAI CIIAFYIASYYNTIMAWALYYLISS FTDQLPWTSCKNSWNTGNCT NYFSEDNITWTLHSTSPAEEFYTRH VLQIHRSKGLQDLGGISWQLALCIM LIFTVIYFSIWKGVKTSGKV VWVTATFPYIILSVLLVRGATLPGA WRGVLFYLKPNWQKLLETGVWIDAA AQIFFSLGPGFGVLLAFASY NKFNNNCYQDALVTSVVNCMTSFVS GFVIFTVLGYMAEMRNEDVSEVAKD AGPSLLFITYAEAIANMPAS TFFAIIFFLMLITLGLDSTFAGLEG VITAVLDEFPHVWAKRRERFVLAVV ITCFFGSLVTLTFGGAYVVK LLEEYATGPAVLTVALIEAVAVSWF YGITQFCRDVKEMLGFSPGWFWRIC WVAISPLFLLFIICSFLMSP PQLRLFQYNYPYWSIILGYCIGTSS FICIPTYIAYRLIITPGTFKERIIK SITPETPTEIPCGDIRLNAVTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: SLC6A4 solute carrier family 6 member 4 [ Homo sapiens (human) ]
• Official Symbol: SLC6A4
• Synonyms: HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
• Gene ID: 6532
• mRNA Refseq: NM_001045.6
• Protein Refseq: NP_001036.1
• MIM: 182138
• UniProt ID: P31645