Active Recombinant Full Length Human SNAP25 Protein, C-Flag-tagged

• Cat.No.: SNAP25-136HFL
• Product Overview: Recombinant Full Length Human SNAP25 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: MS digestion standard
• Molecular Mass: 23.2 kDa
• AA Sequence: MAEDADMRNELEEMQRRADQLADES LESTRRMLQLVEESKDAGIRTLVML DEQGEQLDRVEEGMNHINQD MKEAEKNLKDLGKCCGLFICPCNKL KSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTND ARENEMDENLEQVSGIIGNLRHMAL DMGNEIDTQNRQIDRIMEKADSNKT RIDEANQRATKMLGSGTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport

Gene Information

• Gene Name: SNAP25 synaptosome associated protein 25 [ Homo sapiens (human) ]
• Official Symbol: SNAP25
• Synonyms: SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• Gene ID: 6616
• mRNA Refseq: NM_003081.5
• Protein Refseq: NP_003072.2
• MIM: 600322
• UniProt ID: P60880