Active Recombinant Full Length Human SPP1 Protein, C-Flag-tagged

• Cat.No.: SPP1-91HFL
• Product Overview: Recombinant Full Length Human SPP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Cell treatment
• Molecular Mass: 33.7 kDa
• AA Sequence: MRIAVICFCLLGITCAIPVKQADSG SSEEKQLYNKYPDAVATWLNPDPSQ KQNLLAPQNAVSSEETNDFK QETLPSKSNESHDHMDDMDDEDDDD HVDSQDSIDSNDSDDVDDTDDSHQS DESHHSDESDELVTDFPTDL PATEVFTPVVPTVDTYDGRGDSVVY GLRSKSKKFRRPDIQYPDATDEDIT SHMESEELNGAYKAIPVAQD LNAPSDWDSRGKDSYETSQLDDQSA ETHSHKQSRLYKRKANDESNEHSDV IDSQELSKVSREFHSHEFHS
  HEDMLVVDPKSKEEDKHLKFRISHE LDSASSEVNTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens (human) ]
• Official Symbol: SPP1
• Synonyms: OPN; BNSP; BSPI; ETA-1
• Gene ID: 6696
• mRNA Refseq: NM_001040058.2
• Protein Refseq: NP_001035147.1
• MIM: 166490
• UniProt ID: P10451