Active Recombinant Full Length Human SRPK3 Protein, C-Flag-tagged

• Cat.No.: SRPK3-997HFL
• Product Overview: Recombinant Full Length Human SRPK3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: SRPK3 activity verified in a biochemical assay: SRPK3 (SRSF protein kinase 3) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. SRPK3 is a serine/threonine kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. Varying concentrations of SRPK3 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
• Molecular Mass: 61.8 kDa
• AA Sequence: MSASTGGGGDSGGSGGSSSSSQASC GPESSGSELALATPVPQMLQGLLGS DDEEQEDPKDYCKGGYHPVK IGDVFNGRYHVVRKLGWGHFSTVWL CWDIQRKRFVALKVVKSAGHYTETA VDEIKLLKCVRDSDPSDPKR ETIVQLIDDFRISGVNGVHVCMVLE VLGHQLLKWIIKSNYQGLPVPCVKS IVRQVLHGLDYLHTKCKIIH TDIKPENILLCVGDAYIRRLAAEAT EWQQAGAPPPSRSIVSTAPQEVLQT GKLSKNKRKKMRRKRKQQKR LLEERLRDLQRLEAMEAATQAEDSG LRLDGGSGSTSSSGCHPGGARAGPS PASSSPAPGGGRSLSAGSQT SGFSGSLFSPASCSILSGSSNQRET GGLLSPSTPFGASNLLVNPLEPQNA DKIKIKIADLGNACWVHKHF TEDIQTRQYRAVEVLIGAEYGPPAD IWSTACMAFELATGDYLFEPHSGED YSRDEDHIAHIVELLGDIPP AFALSGRYSREFFNRRGELRHIHNL KHWGLYEVLMEKYEWPLEQATQFSA FLLPMMEYIPEKRASAADCL
  QHPWLNPTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: SRPK3 SRSF protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: SRPK3
• Synonyms: MSSK1; STK23; MSSK-1
• Gene ID: 26576
• mRNA Refseq: NM_014370.4
• Protein Refseq: NP_055185.2
• MIM: 301002
• UniProt ID: Q9UPE1