Active Recombinant Full Length Human ST6GALNAC4 Protein, His tagged

• Cat.No.: ST6GALNAC4-01HFL
• Product Overview: Active recombinant full length human ST6GALNAC4 Protein (Thr36-Arg302) with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell line
• Tag: His
• Protein Length: 1-302 aa
• Description: The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein prefers glycoproteins rather than glycolipids as substrates and shows restricted substrate specificity, utilizing only the trisaccharide sequence Neu5Ac-alpha-2,3-Gal-beta-1,3-GalNAc. In addition, it is involved in the synthesis of ganglioside GD1A from GM1B. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Transcript variants encoding different isoforms have been found for this gene. Readthrough transcripts exist for this gene and the downstream ST6GALNAC6 gene.
• Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum. The specific activity is >1000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 31 kDa
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain at 5 μg per lane.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 centigrade as supplied. 3 months, -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with dry ice or equivalent.
• Reference: 1. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805. 2. Harduin-Lepers, A. et al. (2000) Biochem. J. 352:37. 3. Lee, Y. C. et al. (1999) J. Biol. Chem. 274:11958.
• N-terminal Sequence Analysis: Thr36 and Leu38

Gene Information

• Gene Name: ST6GALNAC4 ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 [ Homo sapiens (human) ]
• Official Symbol: ST6GALNAC4
• Synonyms: ST6GALNAC4; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 4; IV; SIAT3C; SIAT7D; SIAT3-C; SIAT7-D; ST6GalNAc; ST6GALNACIV; alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide alpha-2,6-sialyltransferase; NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-alpha-2, 6-sialyltransferase alpha2,6-sialyltransferase; NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc-alpha-2,6-sialyltransferase IV; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4; ST6 GalNAc alpha-2,6-sialyltransferase 4; ST6 neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 4; sialyltransferase 3C; sialyltransferase 7D ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase); EC 2.4.3.7
• Gene ID: 27090
• mRNA Refseq: NM_175039
• Protein Refseq: NP_778204
• MIM: 606378
• UniProt ID: Q9H4F1