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Active Recombinant Full Length Human TSSK1B Protein, C-Flag-tagged

Cat.No. : TSSK1B-869HFL
Product Overview : Recombinant Full Length Human TSSK1B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.
  • Specification
  • Gene Information
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Description : TSSK1 belongs to a family of serine/threonine kinases highly expressed in testis.
Source : Mammalian cells
Species : Human
Tag : Flag
Form : 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bio-activity : TSSK1B activity verified in a biochemical assay: TSSK1B (testis-specific serine kinase 1B) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. TSSK1B is a serine/threonine kinase that is highly expressed in testis and may be involved in a signaling pathway during male germ cell development or mature sperm function. Varying concentrations of TSSK1B were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Molecular Mass : 41.4 kDa
AA Sequence : MDDAAVLKRRGYLLGINLGEGSYAK VKSAYSERLKFNVAIKIIDRKKAPA DFLEKFLPREIEILAMLNHC SIIKTYEIFETSHGKVYIVMELAVQ GDLLELIKTRGALHEDEARKKFHQL SLAIKYCHDLDVVHRDLKCD NLLLDKDFNIKLSDFSFSKRCLRDD SGRMALSKTFCGSPAYAAPEVLQGI PYQPKVYDIWSLGVILYIMV CGSMPYDDSNIKKMLRIQKEHRVNF PRSKHLTGECKDLIYHMLQPDVNRR LHIDEILSHCWMQPKARGSP SVAINKEGESSRGTEPLWTPEPGSD KKSATKLEPEGEAQPQAQPETKPEG TAMQMSRQSEILGFPSKPST
METEEGPPQQPPETRAQTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining.
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade.
Concentration : >50 ug/mL as determined by microplate BCA method.
Preparation : Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Families : Druggable Genome, Protein Kinase
Gene Name : TSSK1B testis specific serine kinase 1B [ Homo sapiens (human) ]
Official Symbol : TSSK1B
Synonyms : TSK1; TSSK1; FKSG81; SPOGA4; STK22D
Gene ID : 83942
mRNA Refseq : NM_032028.4
Protein Refseq : NP_114417.1
MIM : 610709
UniProt ID : Q9BXA7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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