Active Recombinant Full Length Human TUBB3 Protein, C-Flag-tagged

• Cat.No.: TUBB3-203HFL
• Product Overview: Recombinant Full Length Human TUBB3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Co-immunoprecipitation
• Molecular Mass: 50.3 kDa
• AA Sequence: MREIVHIQAGQCGNQIGAKFWEVIS DEHGIDPSGNYVGDSDLQLERISVY YNEASSHKYVPRAILVDLEP GTMDSVRSGAFGHLFRPDNFIFGQS GAGNNWAKGHYTEGAELVDSVLDVV RKECENCDCLQGFQLTHSLG GGTGSGMGTLLISKVREEYPDRIMN TFSVVPSPKVSDTVVEPYNATLSIH QLVENTDETYCIDNEALYDI CFRTLKLATPTYGDLNHLVSATMSG VTTSLRFPGQLNADLRKLAVNMVPF PRLHFFMPGFAPLTARGSQQ YRALTVPELTQQMFDAKNMMAACDP RHGRYLTVATVFRGRMSMKEVDEQM LAIQSKNSSYFVEWIPNNVK VAVCDIPPRGLKMSSTFIGNSTAIQ ELFKRISEQFTAMFRRKAFLHWYTG EGMDEMEFTEAESNMNDLVS
  EYQQYQDATAEEEGEMYEDDEEESE AQGPKTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection

Gene Information

• Gene Name: TUBB3 tubulin beta 3 class III [ Homo sapiens (human) ]
• Official Symbol: TUBB3
• Synonyms: CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• Gene ID: 10381
• mRNA Refseq: NM_006086.4
• Protein Refseq: NP_006077.2
• MIM: 602661
• UniProt ID: Q13509