| Species : |
Human |
| Source : |
Human |
| Tag : |
His |
| Protein Length : |
1-230 a.a. |
| Description : |
Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. UCHL3 protein is 54% identical to that of UCHL1. |
| Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol. |
| Molecular Weight : |
28.3kDa (250aa), confirmed by MALDI-TOF |
| Purity : |
> 95% by SDS - PAGE |
| Concentration : |
1 mg/ml (determined by Bradford assay) |
| Biological activity : |
Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. |
| Activity Assay : |
1. Prepare a 100ul of recombinant UCH-L3 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC- 96 Well Polystyrene Microplate, black - Fluorescent plate reader |
| Sequences of amino acids : |
MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA |
| Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
