Active Recombinant HIV-1 IIIB p66 (RT) Protein

• Cat.No.: HIV1IIIBp66(RT)-125H

Specification

• Species: HIV
• Source: E.coli
• Tag: Non
• Description: Full length Recombinant heterodimeric HIV-1 IIIB p66 (RT) produced in E.coli expression system. This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA.
• Form: Frozen Liquid
• Bio-activity: 0.1 unit of purified RT incorporates a minimum of 5 picomoles of dTTP per minute under standard RT assay conditions.
• Molecular Mass: 66 kD
• Purity: > 98 % by SDS-PAGE
• Applications: Reverse Trascriptase Reference;
  Diagnostics;
  ELISA / Western ELISA;
  Drug Screening.
• Notes: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
  ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody. Solid phase antigen in 50-100ng amounts shows reactivity with human serum antibodies in dot blot assays.
• Stability: At least 12 months at -75 centigrade.
• Storage: Store at -75 centigrade.
• Dilutions: 50mM Tris,pH8,10% Glycerol Drug Screening, 1mM DTT,0.2M NaCl