Active Recombinant HIV(group M, subtype CRF07_BC) gp140 protein, hFc-tagged

• Cat.No.: gp140-1949H
• Product Overview: Active Recombinant HIV(group M, subtype CRF07_BC) gp140 protein(Ala 29-Ser 661), was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus and a signal peptide at the N-terminus.

Specification

• Species: HIV
• Source: HEK293
• Tag: Fc
• Protein Length: 29-661 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of gp140-Fc bound to human CD4 is 0.3 μM.
• Molecular Mass: The recombinant HIV-1 gp140 consists of 874 amino acids after removal of the signal peptide and has a predicted molecular mass of 98 kDa. The apparent molecular mass of the recombinant protein is approximately 210-230 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.