Active Recombinant HRV 3C Protease, His-tagged

• Cat.No.: 3C Protease-15H
• Product Overview: HRV-3C Protease, His Tag (10 U/μL) is expressed from Human Rhinovirus E. coli. The recombinant enzyme is engineered as a fusion protein with polyhistidine tags, can be easily removed after cleavage.

Specification

• Species: HRV
• Source: E. coli
• Tag: His
• Description: HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.
• Form: Supplied as 0.2 μm filtered solution in 25 mM Tris, 150 mM NaCl with glycerol as protectant.
• Bio-activity: Enzyme Activity: >10 U/μL Unit Definition: One unit will cleave >95% of 100 μg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.
• Purity: >90% as determined by SDS-PAGE.
• Applications: Removal of purification tags from fusion proteins.
• Storage: This product is stable after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 12 months under sterile conditions.
• Concentration: 0.2 mg/mL
• Shipping: This product is supplied and shipped with dry ice, please inquire the shipping cost.

Gene Information

• Official Symbol: 3C Protease
• Synonyms: 3C Protease