| Species : |
HRV |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage. |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
0.6 IU/μl. Unit Definition: One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 90% completion at 4 centigrade in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. |
| Molecular Mass : |
24kDa, observed by reducing SDS-PAGE. |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% by SDS-PAGE analysis. |
| Storage : |
Recombinant PreScission Protease (rPSP) remains stable up to 1 year at -20 centigrade from date of receipt. Please avoid freeze-thaw cycles. |
| Storage Buffer : |
Sterile liquid solution contains 50 mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 50% Glycerol, pH 7.5. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
