Active Recombinant HRV 3C protein, GST-tagged

• Cat.No.: 3C-2843H
• Product Overview: Recombinant HRV 3C protein(), fused with GST tag, was expressed in E.coli.

Specification

• Species: HRV
• Source: E.coli
• Tag: GST
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,1 mM EDTA,0.05% Tween20,1 mM DTT,PH8.0.
• Bio-activity: >1 Units/µg. One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.
• Molecular Mass: This protein carries a GST tag at the N-terminusThe protein has a calculated MW of 46.0 kDa. The protein migrates as 44-46 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.