| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.1mM ZnCl2, 10% Glycerol, pH 7.5. |
| Bio-activity : |
Specific Activity is > 800 pmol/min/μg as measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH |
| AA Sequence : |
QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQM YPLQEIQNLT VKLQLQALQQ NGSSVLSEDK SKRLNTILNT MSTIYSTGKV CNPDNPQECL LLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGV DGYDYSRGQL IEDVEHTFEE IKPLYEHLHA YVRAKLMNAY PSYISPIGCL PAHLLGDMWG RFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKA VCHPTAWDLG KGDFRILMCT KVTMDDFLTA HHEMGHIQYD MAYAAQPFLL RNGANEGFHE AVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQ WMKKWWEMKR EIVGVVEPVP HDETYCDPAS LFHVSNDYSF IRYYTRTLYQ FQFQEALCQA AKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNS FVGWSTDWSP YADQSIKVRI SLKSALGDKA YEWNDNEMYL FRSSVAYAMR QYFLKVKNQM ILFGE EDVRVANLKP RISFNFFVTA PKNVSDIIPR TEVEKAIRMS RSRINDAFRL NDNSLEFLGI QPTLG PPNQPPVSVDHHHHHH |
| Endotoxin : |
Less than 0.1 ng/μg (1 IEU/μg). |
| Purity : |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Storage : |
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
