Active Recombinant Human ACLY Protein, GST-tagged

• Cat.No.: ACLY-420H
• Product Overview: Recombinant Human ACLY fused with GST tag at the N-terminus was produced in Insect cells.

Specification

• Species: Human
• Source: Insect cells
• Tag: GST
• Description: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
• Form: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, and 3 mM DTT.
• Bio-activity: >300pmole/min/ug
• Molecular Mass: 147 kDa
• Purity: >86%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: At least 6 months at -80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein(BSA 0.1-0.5%).

Gene Information

• Gene Name: ACLY ATP citrate lyase [ Homo sapiens ]
• Official Symbol: ACLY
• Synonyms: ACLY; ATP citrate lyase; ATP-citrate synthase; ACL; ATP citrate synthase; ATPCL; CLATP; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
• Gene ID: 47
• mRNA Refseq: NM_001096
• Protein Refseq: NP_001087
• MIM: 108728
• UniProt ID: P53396