Active Recombinant Human ACVR2A protein, hFc-tagged

• Cat.No.: ACVR2A-195H
• Product Overview: Active Recombinant Human ACVR2A protein(Met 1-Pro 134)(NP_001607.1) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-134 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL recombinant Activin A.
• Molecular Mass: The recombinant human ACVR2A/Fc chimera is a disulfide-linked homodimer protein generated after removal of the signal peptide. The monomer comprises 352 amino acids and predicts a molecular mass of 40.0 kDa. As a result of glycosylation, the monomer migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Antibody-based strategies for the detection of Luspatercept (ACE-536) in human serum (2017)
  • Testing for the erythropoiesis-stimulating agent Sotatercept/ACE-011 (ActRIIA-Fc) in serum by means of Western blotting and LC-HRMS (2016)
  • Combined detection of the ActRII‐Fc fusion proteins Sotatercept (ActRIIA‐Fc) and Luspatercept (modified ActRIIB‐Fc) in serum by means of immunoaffinity purification, tryptic digestion, and LC‐MS/MS (2018)

Gene Information

• Gene Name: ACVR2A activin A receptor, type IIA [ Homo sapiens ]
• Official Symbol: ACVR2A
• Synonyms: ACVR2A; activin A receptor, type IIA; activin A receptor, type II , ACVR2; activin receptor type-2A; ACTRII; ACVR2
• Gene ID: 92
• mRNA Refseq: NM_001616
• Protein Refseq: NP_001607
• MIM: 102581
• UniProt ID: P27037

Citation

Antibody-based strategies for the detection of Luspatercept (ACE-536) in human serum.

Journal: Drug testing and analysis    PubMed ID: 28929587    Data: 2018/8/22

Authors: Christian Reichel, Günter Gmeiner, Mario Thevis

Article Snippet:Human recombinant erythropoietins were from Janssen-Cilag (Erypo (epoetin alpha); Vienna, Austria), Shire (Dynepo (epoetin delta); Hampshire, UK), Roche (NeoRecormon (epoetin beta) and MIRCERA; Mannheim, Germany), Amgen (Aranesp (NESP); Thousand Oaks, CA), and ProSpec (recombinant human EPO-alpha/Fc chimera (EPO-Fc); Ness-Ziona, Israel).Human recombinant erythropoietins were from Janssen-Cilag (Erypo (epoetin alpha); Vienna, Austria), Shire (Dynepo (epoetin delta); Hampshire, UK), Roche (NeoRecormon (epoetin beta) and MIRCERA; Mannheim, Germany), Amgen (Aranesp (NESP); Thousand Oaks, CA), and ProSpec (recombinant human EPO-alpha/Fc chimera (EPO-Fc); Ness-Ziona, Israel).. Sotatercept and other ACVR2A-Fc fusion proteins used for cross-reactivity testing were either custom synthesized (Sotatercept; Creative BioMart) or bought from Creative BioMart and R&D Systems (see Table 2).