Active Recombinant Human AGER protein, His-tagged, Low Endotoxin

• Cat.No.: AGER-7322H
• Product Overview: Low Endotoxin Active Recombinant Human AGER protein(Q15109-1)(Ala23- Ala344) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 23-344 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Human AGER, His Tag immobilized on CM5 Chip can bind Human HMGB1, His Tag with an affinity constant of 0.19 μM as determined in SPR assay.
• Molecular Mass: The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: AGER advanced glycosylation end product-specific receptor [ Homo sapiens ]
• Official Symbol: AGER
• Synonyms: AGER; advanced glycosylation end product-specific receptor; RAGE; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta
• Gene ID: 177
• mRNA Refseq: NM_001136
• Protein Refseq: NP_001127
• MIM: 600214
• UniProt ID: Q15109