Active Recombinant Human AKR1B10 protein

• Cat.No.: AKR1B10-406H
• Product Overview: Recombinant Human AKR1B10(1-316aa) was expressed in E. coli.

Specification

• Description: AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer.
• Source: E. coli
• Species: Human
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Bio-activity: Specific activity: approximately 0.32 - 0.4 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.
• Molecular Mass: 36 kDa (316aa), confirmed by MALDI-TOF.
• AA Sequence: MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVRKAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKPVTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARIVENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Tag: Non
• Protein length: 1-316

Gene Information

• Gene Name: AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase) [ Homo sapiens ]
• Official Symbol: AKR1B10
• Synonyms: AKR1B10; aldo-keto reductase family 1, member B10 (aldose reductase); AKR1B11; aldo-keto reductase family 1 member B10; AKR1B12; aldo keto reductase family 1; member B11 (aldose reductase like); aldose reductase like 1; aldose reductase like peptide; aldose reductase related protein; ALDRLn; ARL 1; ARL1; HIS; HSI; small intestine reductase; ARP; hARP; SI reductase; aldose reductase-like 1; aldose reductase-like peptide; aldose reductase-related protein; aldo-keto reductase family 1, member B11 (aldose reductase-like); ARL-1; MGC14103
• Gene ID: 57016
• mRNA Refseq: NM_020299
• Protein Refseq: NP_064695
• MIM: 604707
• UniProt ID: O60218
• Chromosome Location: 7q33
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: aldo-keto reductase (NADP) activity; geranylgeranyl reductase activity; indanol dehydrogenase activity; protein binding; retinal dehydrogenase activity

Reviews

09/20/2022

Has therapeutic effects on specific disease models and is a promising candidate for new drugs.

03/31/2022

The laboratory personnel feedback that the protein is easy to operate, can meet the experimental needs, and has a good cost performance.

05/30/2019

It has been found to increase yield and purity and improve production efficiency in biotechnology production.

09/30/2018

Good fluorescence labeling performance in light microscopy technology, which is helpful for cell imaging studies.

Q&As

What are the methods for laboratory detection of AKR1B10 protein?

AKR1B10 protein is mainly detected in laboratory by enzyme-linked immunosorbent assay, fluorescence quantitative PCR and other methods.

Which cell signaling pathways does AKR1B10 protein participate in?

AKR1B10 is involved in a variety of cell signaling pathways, such as NF-κB, WNT/ β-catenin signaling pathways, and thus plays a role in cell proliferation, differentiation and apoptosis.

How does AKR1B10 play a role in neuroprotective mechanisms?

This protein is involved in the NADP/NADPH metabolic pathway, plays an antioxidant and neuroprotective role, and thus has a promising application in the prevention and treatment of central nervous system diseases.

AKR1B10 has the potential to be used in the early diagnosis and prevention of which cancers?

AKR1B10 has a certain application prospect in the early diagnosis and prognosis evaluation of lung cancer, colorectal cancer, liver cancer and other cancers.

How is AKR1B10 protein distributed in the human body

The AKR1B10 protein is an alcohol contraction enzyme that is associated with a variety of diseases and is mainly found in tissues such as liver, lung, stomach, breast, kidney, pancreas and intestine.

Does AKR1B10 have potential applications in the treatment of inflammatory diseases?

AKR1B10 inhibitors can be used to prevent and treat a variety of inflammatory diseases, such as rheumatoid arthritis and hepatitis B.

How does AKR1B10 function in lung cancer?

AKR1B10 protein is associated with cell proliferation, angiogenesis and malignant metastasis in lung cancer

In what diseases do AKR1B10 inhibitors have potential applications?

AKR1B10 inhibitors have potential anti-tumor and anti-inflammatory therapeutic effects and can be used in the treatment of lung cancer, breast cancer, diabetes and other diseases.

Whether AKR1B10 protein is involved in tumorigenesis?

The expression level of AKR1B10 protein in tumor cells is increased, which is related to tumor proliferation, invasion and metastasis, and is a new tumor biomarker.

What is the role of AKR1B10 protein in metabolic diseases?

AKR1B10 is involved in the occurrence and development of metabolic diseases, such as diabetes and obesity, and is a possible therapeutic target.

Whether AKR1B10 protein has a function in the human body

AKR1B10 protein is involved in various metabolic processes and cell proliferation and growth, such as steroid hormone metabolism, fatty acid synthesis, cell cycle regulation and so on.

Does there any steroid hormone metabolic pathways involved in AKR1B10?

The metabolic pathways of steroid hormones involved in AKR1B10 include oxidative metabolism and reductive metabolism of steroid hormones.

Is AKR1B10 protein associated with hepatotoxicity?

AKR1B10 protein is involved in the metabolism of some drugs and may be related to hepatotoxicity, which is a hot molecule in the study of drug metabolism and toxicity.

What diseases is AKR1B10 associated with?

AKR1B10 protein is associated with a variety of diseases, such as tumors, lung diseases, metabolic diseases, and neurological diseases.

In what retinal diseases is AKR1B10 associated?

AKR1B10 protein is involved in the metabolism of retinal glycoglycation end products, which is associated with diabetic retinopathy and other diseases.