Active Recombinant Human AKR1B10 protein

• Cat.No.: AKR1B10-406H
• Product Overview: Recombinant Human AKR1B10(1-316aa) was expressed in E. coli.

Specification

• Description: AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer.
• Source: E. coli
• Species: Human
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Bio-activity: Specific activity: approximately 0.32 - 0.4 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.
• Molecular Mass: 36 kDa (316aa), confirmed by MALDI-TOF.
• AA Sequence: MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVRKAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKPVTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARIVENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase) [ Homo sapiens ]
• Official Symbol: AKR1B10
• Synonyms: AKR1B10; aldo-keto reductase family 1, member B10 (aldose reductase); AKR1B11; aldo-keto reductase family 1 member B10; AKR1B12; aldo keto reductase family 1; member B11 (aldose reductase like); aldose reductase like 1; aldose reductase like peptide; aldose reductase related protein; ALDRLn; ARL 1; ARL1; HIS; HSI; small intestine reductase; ARP; hARP; SI reductase; aldose reductase-like 1; aldose reductase-like peptide; aldose reductase-related protein; aldo-keto reductase family 1, member B11 (aldose reductase-like); ARL-1; MGC14103
• Gene ID: 57016
• mRNA Refseq: NM_020299
• Protein Refseq: NP_064695
• MIM: 604707
• UniProt ID: O60218
• Chromosome Location: 7q33
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: aldo-keto reductase (NADP) activity; geranylgeranyl reductase activity; indanol dehydrogenase activity; protein binding; retinal dehydrogenase activity