Active Recombinant Human ALCAM protein, hFc&His-tagged

• Cat.No.: ALCAM-2650H
• Product Overview: Active Recombinant Human ALCAM/CD166 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Ala526) of human ALCAM/CD166 (Accession #NP_001618.2) fused with an Fc, 6×His tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc&His
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA.Immobilized Human CD6 at 1 μg/mL (100 μL/well) can bind Human CD166 with a linear range of 0.05-1.2 ng/mL.
  2.Measured by its ability to block adhesion of Jurkat cells to immobilized Recombinant Human CD6 Protein . The ED50 for this effect is 2.7-10.8 μg/mL.
• Molecular Mass: 120 kDa
• Protein Length: Met1-Ala526
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 97% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: ALCAM activated leukocyte cell adhesion molecule [ Homo sapiens ]
• Official Symbol: ALCAM
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; activated leucocyte cell adhesion molecule; CD166 antigen; CD166; MEMD; FLJ38514; MGC71733
• Gene ID: 214
• mRNA Refseq: NM_001243280
• Protein Refseq: NP_001230209
• MIM: 601662
• UniProt ID: Q13740