Active Recombinant Human APP protein, His & GST-tagged

• Cat.No.: APP-192H
• Product Overview: Active Recombinant Human APP protein(Asp 672-Val 711)(P05067-1), corresponding to the Beta-amyloid protein 40, was expressed in E. coli, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: E. coli
• Tag: GST&His
• Protein Length: 672-711 aa
• Form: Lyophilized from sterile 50mM Tris, 500mM NaCl, pH 7.5.
• Bio-activity: Measured by its ability to bind biotinylated recombinant human AGER in a functional ELISA.
• Molecular Mass: The recombinant human Beta-APP40/GST chimera consists of 278 amino acids and has a calculated molecular mass of 31.8 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: APP amyloid beta (A4) precursor protein [ Homo sapiens ]
• Official Symbol: APP
• Synonyms: APP; amyloid beta (A4) precursor protein; AD1, Alzheimer disease; amyloid beta A4 protein; peptidase nexin II; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Gene ID: 351
• mRNA Refseq: NM_000484
• Protein Refseq: NP_000475
• MIM: 104760
• UniProt ID: P05067