Active Recombinant Human B4GALT1 protein, His-tagged

• Cat.No.: B4GALT1-646H
• Product Overview: Recombinant Human B4GALT1 protein (NP_001488.2) (Gly 44-Ser 398) was fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gly 44-Ser 398
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: B4GALT1 treated IgG1 Fc showed a significantly higher proportion of galactosylated component (G2 component) compared with the untreated sample (78.27% vs 9.34%).
• Molecular Mass: The secreted recombinant human B4GALT1 consists of 371 amino acids and predicts a molecular mass of 41.5 kDa. By SDS-PAGE under reducing conditions, rh B4GALT1 migrates as an approximately 45-55 kDa protein due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 92 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Gene Information

• Gene Name: B4GALT1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 [ Homo sapiens ]
• Official Symbol: B4GALT1
• Synonyms: B4GALT1; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1; GGTB2; beta-1,4-galactosyltransferase 1; beta4Gal T1; lactose synthase; beta-1,4-GalTase 1; glycoprotein-4-beta-galactosyltransferase 2; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1; GT1; GTB; CDG2D; B4GAL-T1; beta4Gal-T1; MGC50983; DKFZp686N19253
• Gene ID: 2683
• mRNA Refseq: NM_001497
• Protein Refseq: NP_001488
• MIM: 137060
• UniProt ID: P15291