Active Recombinant Human BAI1 Protein, His-tagged

• Cat.No.: BAI1-921H
• Product Overview: Recombinant Human BAI1 (Accession # O14514) Ala31-Thr879, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala31
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.When 4 x 10^4 cells per well are added to rhBAI1 coated plates (0.5 µg/mL, 100 µL/well), approximately 40%-70% will adhere after 1 hour at 37° C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human BAI1, His-tagged has a calculated MW of 93.6 kDa. In SDS-PAGE migrates as 105-115 kDa, reducing conditions .
• Protein length: Ala31-Thr879
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: BAI1 brain-specific angiogenesis inhibitor 1 [ Homo sapiens ]
• Official Symbol: BAI1
• Synonyms: BAI1; brain-specific angiogenesis inhibitor 1; GDAIF; FLJ41988
• Gene ID: 575
• mRNA Refseq: NM_001702
• Protein Refseq: NP_001693
• MIM: 602682
• UniProt ID: O14514
• Chromosome Location: 8q24.3