Active Recombinant Human BCL2 protein, His-tagged

• Cat.No.: BCL2-267H
• Product Overview: Active Recombinant Human BCL2 protein(P10415-) (Met 1-Asp 211) was expressed in E.coli, with a polyhistide tag at the C-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-211 aa
• Form: Lyophilized from sterile 50mM Tris, 20% glycerol, 100mM Arg, pH 8.5.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human BCL2-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse BCL2L1-His, The EC50 of biotinylated mouse BCL2L1-His is 0.07-0.15 μg/ml.
• Molecular Mass: The recombinant human BCL2 consisting of 221 amino acids and has a calculated molecular mass of 24.7 kDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BCL2 B-cell CLL/lymphoma 2 [ Homo sapiens ]
• Official Symbol: BCL2
• Synonyms: BCL2; B-cell CLL/lymphoma 2; apoptosis regulator Bcl-2; Bcl 2; PPP1R50; protein phosphatase 1; regulatory subunit 50; protein phosphatase 1, regulatory subunit 50; Bcl-2
• Gene ID: 596
• mRNA Refseq: NM_000633
• Protein Refseq: NP_000624
• UniProt ID: P10415