Active Recombinant Human BDNF Protein (His132-Arg250), C-His tagged, Animal-free, Carrier-free

• Cat.No.: BDNF-266H
• Product Overview: Recombinant Human BDNF Protein (His132-Arg250) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Brain-derived neurotrophic factor (BDNF) is a member of neurotrophin family that not only primarily expressed in hippocampus, amygdala, cerebral cortex, hypothalamus and cerebellum but also has been detected in blood platelets and in circulating plasma. BDNF is a 27.8 kDa protein containing 247 residues, which plays a critical role in regulating synaptic transmission and plasticity in various region of the CNS. Additionally, BNDF can acts as a modulator in the long-term potentiation of memory-related modifications in hippocampal synaptic transmission.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED50 for this effect is < 2 ng/mL.
• Molecular Mass: The protein has a calculated MW of 14.45 kDa.
  The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: His132-Arg250
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: BDNF brain-derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: BDNF
• Synonyms: BDNF; brain-derived neurotrophic factor; neurotrophin; abrineurin; MGC34632
• Gene ID: 627
• mRNA Refseq: NM_001143805
• Protein Refseq: NP_001137277
• MIM: 113505
• UniProt ID: P23560

Reviews

12/31/2022

The BDNF protein is carefully characterized, and its functional properties are well-documented.

04/08/2021

With its high purity and bioactivity, BDNF protein ensures reliable and consistent results in a wide range of experimental applications.

11/27/2017

The BDNF protein is of exceptional quality and is perfectly suited to meet the experimental needs of researchers.

Q&As

How is BDNF measured in clinical settings?

BDNF levels can be measured through blood tests or cerebrospinal fluid analysis, providing insights into the protein's concentration in the body.

What neurological disorders are associated with BDNF deficiency?

BDNF deficiency has been linked to conditions such as Alzheimer's disease, Parkinson's disease, and depression.

Are there medications that target BDNF for clinical use?

While there are no direct BDNF medications, certain drugs and treatments aim to enhance BDNF levels indirectly, offering potential therapeutic benefits.

How does physical exercise influence BDNF levels, and what implications does this have for clinical applications?

Physical exercise has been shown to increase BDNF production, suggesting that exercise could be used as a non-pharmacological intervention to support neurological health.

What role does BDNF play in synaptic plasticity, and how does it impact learning and memory?

BDNF is crucial for synaptic plasticity, influencing the strength and efficacy of connections between neurons, which is vital for learning and memory processes.