Active Recombinant Human BLK protein, GST-tagged

• Cat.No.: BLK-953H
• Product Overview: Active Recombinant Human BLK protein(NP_001706.2) (Met 1-Pro 505) was expressed in Insect Cells, fused with the GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-505 aa
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 5mM GSH, pH 7.4.
• Bio-activity: The specific activity was determined to be 17.4 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.
• Molecular Mass: The recombinant human BLK/GST chimera consists of 730 amino acids and migrates as an approximatly 84 kDa band as predicted in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BLK B lymphoid tyrosine kinase [ Homo sapiens ]
• Official Symbol: BLK
• Synonyms: BLK; B lymphoid tyrosine kinase; tyrosine-protein kinase Blk; MGC10442; p55-Blk; b lymphocyte kinase; BLK nonreceptor tyrosine kinase; MODY11
• Gene ID: 640
• mRNA Refseq: NM_001715
• Protein Refseq: NP_001706
• MIM: 191305
• UniProt ID: P51451