Active Recombinant Human BMPR2 protein, His&hFc-tagged

• Cat.No.: BMPR2-133H
• Product Overview: Active Recombinant Human BMPR2 protein(NP_001195.2)(Met 1-Ile 151) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-151 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human BMPR-II-Fc at 10 μg/mL (100 μl/well) can bind biotinylated human BMP2-Fc, The EC50 of biotinylated human BMP2-Fc is 80-110 ng/mL.
• Molecular Mass: The recombinant human BMPR-II/Fc is a disulfide-linked homodimer. The reduced monomer consists of 373 amino acids and has a predicted molecular mass of 42 kDa. As a result of glycosylation, the apparent molecular mass of rh BMPR-II/Fc monomer migrates with an apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BMPR2 bone morphogenetic protein receptor, type II (serine/threonine kinase) [ Homo sapiens ]
• Official Symbol: BMPR2
• Synonyms: BMPR2; bone morphogenetic protein receptor, type II (serine/threonine kinase); PPH1, primary pulmonary hypertension 1; bone morphogenetic protein receptor type-2; BMPR II; BMPR3; BRK 3; T ALK; BMPR-2; BMP type-2 receptor; BMP type II receptor; type II activin receptor-like kinase; bone morphogenetic protein receptor type II; type II receptor for bone morphogenetic protein-4; BMR2; PPH1; BRK-3; T-ALK; BMPR-II; FLJ41585; FLJ76945
• Gene ID: 659
• mRNA Refseq: NM_001204
• Protein Refseq: NP_001195
• MIM: 600799
• UniProt ID: Q13873