Active Recombinant Human Bone Morphogenetic Protein 15

• Cat.No.: BMP15-579H
• Product Overview: Recombinant Human BMP15protein was expressed in Chinese Hamster Ovary cell line. Gln268-Arg392.

Specification

• Cat. No.: BMP15-579H
• Description: Bone morphogeneticprotein 15 (BMP15), also known as GDF9B, is a TGFβ superfamily ligand that isexpressed by oocytes throughout folliculogenesis, and plays an important rolein oocyte development. BMP15 promotes the FSHindependent proliferation ofovarian granulosa cells (GC) and induces GC glycolysis and cholesterolsynthesis. It also induces GC production of stem cell factor which, in turn,negatively regulates BMP15 expression in oocytes.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilized from a0.2 μm filtered solution in HCl with BSA as a carrier protein.
• N-terminalSequence: Gln268
• Molecular Weight: 13.9 kDa
• Activity: Measured by itsability to induce Smad3 phosphorylation in P19 mouse embryonal carcinomacells. Approximately 10 ng/mL can effectively induce Smad3 phosphorylation.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 15 kDa,reducing conditions.
• Purity: > 90%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mL in sterile4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: BMP15 bone morphogenetic protein15 [ Homo sapiens ]
• Official Symbol: BMP15
• Synonyms: BMP15; bonemorphogenetic protein 15; ODG2; POF4; GDF9B; BMP-15; GDF-9B;growth/differentiation factor 9B
• Gene ID: 9210
• mRNA Refseq: NM_005448
• Protein Refseq: NP_005439
• MIM: 300247
• UniProt ID: O95972
• Chromosome Location: Xp11.2
• Function: cytokine activity;growth factor activity