Active Recombinant Human BPI, Fc Chimera

• Cat.No.: BPI-175H
• Product Overview: Recombinant Human BPI (Accession # AAA51841) was produced in Human embryonic kidney cell, HEK293-derived.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Predicted N Terminal: Val32
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit LPS-induced IL-8 secretion by THP-1 human acute monocytic leukemia cells. Wilde, C.G. et al. (1994) J. Biol. Chem. 269:17411.The ED50 for this effect is typically 0.4-2.4 ng/mL.
• Molecular Mass: Recombinant Human BPI, Fc Chimera has a calculated MW of 77.3 kDa (monomer). In SDS-PAGE migrates as 75-85 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: BPI bactericidal/permeability-increasing protein [ Homo sapiens ]
• Official Symbol: BPI
• Synonyms: BPI; bactericidal/permeability-increasing protein; bactericidal permeability-increasing protein; BPI fold containing family D; member 1; BPIFD1; CAP 57; recombinant BPI holoprotein, rBPI; BPI fold containing family D, member 1; rBPI
• Gene ID: 671
• mRNA Refseq: NM_001725
• Protein Refseq: NP_001716
• MIM: 109195
• UniProt ID: P17213
• Chromosome Location: 20q11.23