Species : |
Human |
Source : |
Sf9 Cells |
Tag : |
GST&His |
Protein Length : |
417-766 a.a. |
Description : |
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
Form : |
In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol) |
Bio-activity : |
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG).Substrate : MAP2K1 [inactive mutant]. ATP: 100 µM. |
Molecular Mass : |
69.127 kDa |
Quality Control Test : |
2 ug/lane SDS-PAGE Stained with Coomassie Blue |
Notes : |
Result of activity analysis |
Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Concentration : |
0.294 ug/uL |
Storage Buffer : |
In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol) |